graph pad prism version 10.1.0 (264) software Search Results


10-(tert-butoxy)-10-oxodecanoic acid  (MuseChem Chemicals)
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10-(tert-Butoxy)-10-oxodecanoic acid（Cat No.:L026491）is a specialized chemical compound utilized in advanced organic synthesis and pharmaceutical research. This compound features a tert-butoxy group and a keto function on a decanoic acid backbone, providing unique reactivity and versatility
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cynomolgus monkey cxcl10 (ip-10) polyclonal antibody  (Kingfisher Biotech)
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Anti-cynomolgus monkey CXCL10 polyclonal antibody (catalog KP1264Y) is made in rabbits, affinity-purified by cynomolgus monkey CXCL10 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant cynomolgus monkey CXCL10 (immunogen) and
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raw 264 7 cells  (Addgene inc)
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Addgene inc raw 264 7 cells
A RAW264.7 cells were transfected with an NF-κB luciferase reporter. 14 hours post-transfection these cells were infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours, the infection time yielding robust NF-κB-luciferase activity (Supplemental Fig. 1). Luciferase activity was measured by a luminometer and values converted into percent increase over matched resting/uninfected samples. B PBM were infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blotting was done on cell lysates to measure phospho-GSK3α/β, then reprobed with anti-actin as a loading control. C Matched cell lysates from Figure 5B were tested by western blotting for phospho-GSK3β, then reprobed for total GSK3β as a loading control. D PBM were treated with DMSO vehicle control or the LY294002 for 30 minutes, and then infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blots were done on cell lysates for phospho-GSK3α/β, followed by reprobing for actin as a loading control. E RAW264.7 cells were transfected with an NF-κB luciferase reporter plasmid. Transfected cells were pretreated with DMSO vehicle control or SB-216763 GSK3β inhibitor for 30 minutes, then infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours. Cells were lysed and luciferase activity measured by a luminometer. Data are expressed as percent increase in activity over matched uninfected control. F RAW 264.7 cells were pre-treated with DMSO vehicle control or SB-216763, and then infected with B. cenocepacia (B.c.) at an MOI of 5 for 8 hours. Cleared cell lysates were assayed for TNFα production by ELISA. Data represent the average of 3 samples and error bars denote standard deviation. * denotes a p value < 0.05 from Student’s t-test.
Raw 264 7 Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/graph+pad+prism+version+10%2E1%2E0+(264)+software/pCBASceI+(Plasmid+%2326477)/pmc03131456-70-29-18
Average 96 stars, based on 1 article reviews
raw 264 7 cells - by Bioz Stars, 2026-09
96/100 stars
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sr 2640 hydrochloride  (Toronto Research Chemicals)
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5,10,15-tris(4-bromophenyl)corrole  (BOC Sciences)
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triptycene-9,10-dicarboxaldehyde  (Aladdin Scientific Corporation)
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fam26f calhm6 nm 001010919 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles FAM26F Myc DDK tagged Human family with sequence similarity 26 member F FAM26F 200ul 10 7 TU mL
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ngal antibody  (Fitzgerald Industries International)
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Mouse monoclonal NGAL antibody
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recombinant human cyp4f22 protein  (Novus Biologicals)
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The Recombinant Human CYP4F22 Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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ngal antibody  (Biosynth Carbosynth)
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Mouse monoclonal NGAL antibody
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trans-(±)-4-(4-methoxyphenyl)-1-methyl-3-piperidinemethanol  (Toronto Research Chemicals)
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A RAW264.7 cells were transfected with an NF-κB luciferase reporter. 14 hours post-transfection these cells were infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours, the infection time yielding robust NF-κB-luciferase activity (Supplemental Fig. 1). Luciferase activity was measured by a luminometer and values converted into percent increase over matched resting/uninfected samples. B PBM were infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blotting was done on cell lysates to measure phospho-GSK3α/β, then reprobed with anti-actin as a loading control. C Matched cell lysates from Figure 5B were tested by western blotting for phospho-GSK3β, then reprobed for total GSK3β as a loading control. D PBM were treated with DMSO vehicle control or the LY294002 for 30 minutes, and then infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blots were done on cell lysates for phospho-GSK3α/β, followed by reprobing for actin as a loading control. E RAW264.7 cells were transfected with an NF-κB luciferase reporter plasmid. Transfected cells were pretreated with DMSO vehicle control or SB-216763 GSK3β inhibitor for 30 minutes, then infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours. Cells were lysed and luciferase activity measured by a luminometer. Data are expressed as percent increase in activity over matched uninfected control. F RAW 264.7 cells were pre-treated with DMSO vehicle control or SB-216763, and then infected with B. cenocepacia (B.c.) at an MOI of 5 for 8 hours. Cleared cell lysates were assayed for TNFα production by ELISA. Data represent the average of 3 samples and error bars denote standard deviation. * denotes a p value < 0.05 from Student’s t-test.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Akt-mediated pro-inflammatory response of mononuclear phagocytes infected with Burkholderia cenocepacia occurs by a novel GSK3?-dependent, IKK-independent mechanism

doi: 10.4049/jimmunol.1003034

Figure Lengend Snippet: A RAW264.7 cells were transfected with an NF-κB luciferase reporter. 14 hours post-transfection these cells were infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours, the infection time yielding robust NF-κB-luciferase activity (Supplemental Fig. 1). Luciferase activity was measured by a luminometer and values converted into percent increase over matched resting/uninfected samples. B PBM were infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blotting was done on cell lysates to measure phospho-GSK3α/β, then reprobed with anti-actin as a loading control. C Matched cell lysates from Figure 5B were tested by western blotting for phospho-GSK3β, then reprobed for total GSK3β as a loading control. D PBM were treated with DMSO vehicle control or the LY294002 for 30 minutes, and then infected with B. cenocepacia for 20, 40, or 60 minutes. ‘R’ represents uninfected cells. Western blots were done on cell lysates for phospho-GSK3α/β, followed by reprobing for actin as a loading control. E RAW264.7 cells were transfected with an NF-κB luciferase reporter plasmid. Transfected cells were pretreated with DMSO vehicle control or SB-216763 GSK3β inhibitor for 30 minutes, then infected with B. cenocepacia (B.c.) at an MOI of 5 for 5 hours. Cells were lysed and luciferase activity measured by a luminometer. Data are expressed as percent increase in activity over matched uninfected control. F RAW 264.7 cells were pre-treated with DMSO vehicle control or SB-216763, and then infected with B. cenocepacia (B.c.) at an MOI of 5 for 8 hours. Cleared cell lysates were assayed for TNFα production by ELISA. Data represent the average of 3 samples and error bars denote standard deviation. * denotes a p value < 0.05 from Student’s t-test.

Article Snippet: Transfection 5 μg of pcDNA vector with or without wild-type human GSK3β, Addgene plasmid 14753 ( 24 ), (Addgene, Cambridge, MA) was used for each transfection with 10×10 6 RAW 264.7 cells.

Techniques: Transfection, Luciferase, Infection, Activity Assay, Western Blot, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Standard Deviation